Journal: Vaccines

Article Title: Development of a Macrophage-Based ADCC Assay

doi: 10.3390/vaccines9060660

Figure Lengend Snippet: Antibodies used in this study.

Article Snippet: Trastuzumab and Rituximab antibodies were purchased from Invivogen: anti-HER2-Tra-hIgG1 (her2tra-mab1), anti-hCD20-hIgG1 (hcd20-mab1), anti-hCD20-hIgG1NQ (hcd20-mab12), anti-hCD20-hIgG2 (hcd20-mab2), anti-hCD20-hIgG3 (hcd20-mab3), anti-hCD20-IgG4 (hcd20-mab4), anti-hCD20-mIgG1 (hcd20-mab9), and anti-CD20-mIgG2a (hcd20-mab10). mIgG2b isotype control clone MPC-11 (559530) was purchased from BD.

Techniques:

Journal: Vaccines

Article Title: Development of a Macrophage-Based ADCC Assay

doi: 10.3390/vaccines9060660

Figure Lengend Snippet: Antibodies used in this study.

Article Snippet: Rituximab , CD20 , mIgG1 , Invivogen.

Techniques:

Journal: PLoS ONE

Article Title: Natural killer cell phenotype is altered in HIV-exposed seronegative women

doi: 10.1371/journal.pone.0238347

Figure Lengend Snippet: (A) Background-subtracted frequency of cells positive for CD107a, IFN-γ, and TNF-ɑ in NK cells from healthy (n = 7) and HESN (n = 10) donors in an in vitro Rituximab-mediated ADCC assay with CD20 + Raji target cells. Exact p-values by unpaired Wilcoxon signed-rank test are shown for each plot. (B) Pearson correlation between percentage of CD16 hi cells (in the CyTOF profiling of NK cells) and each of the functional markers CD107a, IFN-γ, and TNF-ɑ (in the in vitro ADCC assay) for each donor in both healthy (n = 7) and HESN (n = 10) groups combined. Pearson correlation coefficient (R) and exact p-value is shown for each plot.

Article Snippet: 1x10 5 NK cells were mixed with 4x10 5 CD20 + Raji cells (ATCC CCL-86, used at passage 9–11), in the presence or absence of 1μg/ml Rituximab (non-fucosylated human CD20 IgG1 antibody, Invivogen, San Diego, CA, USA).

Techniques: In Vitro, ADCC Assay, Functional Assay